Background: Breast cancer is the most common cancer in women. Histopathology\nplays an important part in determining the treatment strategy for\nwomen with breast cancer. GSTP1 plays an important role in protecting cells\nfrom cytotoxic and carcinogenic agents and it is expressed in normal tissues at\nvariable levels in different cell types. CDH1 plays a critical role for establishment\nand maintenance of polarity and differentiation of epithelium during\nthe development period. Also, it plays an important role in signal transduction,\ndifferentiation, gene expression, cellmotility and inflammations. Methods:\nIn this study the promoter methylation levels of GSTP1 and CDH1\ngene which are associated with breast cancer were investigated by technique\nof Methylation Sensitive High Resolution Melting Analysis (MS-HRM). We\nanalysed primary tumor core biopsies from 80 high-risk primary breast cancer\npatients (tumors � 2 cm and/or lymphatic metastase and/or distant metastases\nand/or under 40 years). Also the patients� histopathologic types were associated\nwith the methylation levels. Results: In our study the promoter hypermethylation\nstatus was observed at different rates; GSTP1 and CDH1 hypermethylation\nfrequencies were 82% and 95% respectively. The promoter\nhypermethylation levels of the genes were found to be significant with lymph\nnode positivity, ER positivity and HER2/neu negativity. Conclusion: Our\nstudy is important as being the first study that analyzes association between\nhistopathologic type and GSTP1 and CDH1 gene promotor methylation status\nin Turkish population.
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